Adelaide Proteomics Centre

The Adelaide Proteomics Centre (APC) at the University of Adelaide offers researchers from all fields of biological sciences a state-of-the-art proteomics facility.

Its combination of internationally recognised expertise, advanced equipment and customer service focus is unique.

Proteomics services

The APC is equipped to provide expert services in mass spectrometry based protein identification, quantitation, characterisation of post-translational modifications, such as glycosylation and phosphorylation.

We also provide expert 2D DIGE characterisation of protein expression, N-terminal sequencing, and MALDI imaging mass spectrometry.

We can offer standard and custom services for proteomics, high throughput screening and quality control of small molecules to researchers and industry.

  • Mass spectrometry

    The APC is equipped with the following equipment:

    • Orbitrap LTQ XL ETD (Thermo Fisher Scientific),
    • Impact II™ QTOF (Bruker Daltonics),
    • amaZon ETD (Bruker Daltonics),
    • HCT Ultra Ion Trap (Bruker Daltonics),
    • ultrafleXtreme and ultraflex III MALDI-TOF/TOFs (Bruker Daltonics).

    We offer the following mass spectrometry based analytical workflows as standard services:

    • Protein identification by Mass Spectrometry (Peptide Sequencing)
    • Label and label free quantification of complex protein mixtures
    • Targeted quantification of peptides/proteins
    • MALDI Imaging
    • Biomolecule Mass Measurement

    Our team of experts also offers custom analyses, high throughput screening and QC services on arrangement.

  • Electrophoresis

    2-DE provides a method for separating complex mixtures based on two intrinsic properties of proteins: their isoelectric points and molecular weights.

    In the first dimension, termed isoelectric focusing (IEF), the proteins are separated out on a thin strip of polyacrylamide gel according to a specified pH gradient. The length of the strip and the range of the immobilised pH gradient (IPG) can be chosen to suit different types of samples.

    In the second dimension the proteins are separated according to their molecular weight. The resulting 2-DE can be stained or transferred for blotting applications.

    Specialised visualisation techniques can be used to detect changes in protein expression between two samples, referred to as Difference In-Gel Electrophoresis (DIGE) or to investigate certain post-translational modifications.

    The APC has two IEF platforms for separation in the first dimension: an IPGphor II and Multiphor II. Depending on the strip length, SDS-PAGE in the second dimension can be performed using the Ettan DALTtwelve system (capacity: 12 large-format gels), the HPE Tower (capacity: 4 large-format gels) the SE 600 Ruby system (capacity: 6 medium-format gels) or the miniVE system (capacity: 6 mini-gels).

    If required, electron-transfer can be carried out for large, medium and mini-gels with the TE77 PWR semi-dry apparatus.

Contact us

If you would like to carry out any of our services, discuss the capabilities in further detail, including methodologies, or simply explore the possibilities available please contact us.

The Adelaide Proteomics Centre is located on Level 1 of the Molecular Life Sciences Building at the University of Adelaide's North Terrace campus.

Delivery address

Adelaide Proteomics Centre
Molecular Life Sciences Building
The University of Adelaide
via gate 8, Victoria Drive
Adelaide, South Australia 5005

Postal address

Adelaide Proteomics Centre
C/- School of Biological Sciences
University of Adelaide
Adelaide, South Australia 5005