How Shigella initiates infection
Investigate the Shigella adherence to and invasion of the human colon.
The project will focus on the interaction of Shigella flexneri bacteria with human colon cells to study genes and proteins that potentially affect cell adherence and invasion. A variety of advanced human tissue and cell culture based infection model systems will be used.
Students will gain exposure to a variety of methods including gene cloning, PCR, DNA sequencing, site-directed mutagenesis, bacterial genetics, gel electrophoresis; protein purification, tissue cell culture; fluorescence and confocal microscopy. First-hand experience in experimental design and interpretation of results will be possible.
It is expected that the results of experimental work will lead to a publication.