Characterising the jasmonate hormones in barley, and how they affect beer
Investigate the biochemistry and function of barley plant hormones
Hormones, such as jasmonates, drive many physiological processes during plant development and response to environmental stresses. In barley, jasmonates also regulate the lipoxygenase (lox) pathway, which can affect beer taste and foaming.
This project will:
- Identify genes involved in jasmonate biosynthesis from the recently sequenced barley genome
- Determine their spatial and temporal patterns of expression from a new barley tissue series using quantitative PCR (qPCR)
- Examine biochemical profiles (LC/MS) of barley, malt and spent grain samples from a brewery to find jasmonate products
- Develop a new barley tissue series using lox mutant lines to examine changes jasmonate biosynthetic gene transcription
You will develop skills in:
- Plant tissue harvesting and sample preparation
- Transcriptome analysis
- LC/MS data analysis
Wasternack, C. and Parthier, B., 1997. Jasmonate-signalled plant gene expression. Trends in Plant Science, 2(8), pp.302-307.