Discovery of new genes that promote T cell activity against cancer
The Chemokine Biology Lab has honours projects available with the aim to identify novel genes involved in T cell trafficking to solid tumours.
T cells have the capacity to eliminate tumour cells, but this often fails in solid tumours as T cell trafficking into the tumour is limited. In this project, we will utilise genome-wide transcriptional activation libraries to identify novel genes that can enhance T cell recruitment into solid tumours.
This will involve transducing activated T cells with viral vectors encoding a genome-wide transcriptional activation library and then performing in vitro chemotaxis assays towards tumours or in vivo transfer into tumour bearing mice.
T cells that migrate to tumours will then be harvested and subject to Next Generation Sequencing to identify the upregulated genes that are present in T cells that invade the tumour. These genes will then be selected for independent validation and tested for their ability to enhance CAR T cell recruitment to solid tumours.
The project will involve T cell culture, mouse models of solid human cancers, molecular biology, Next Generation Sequencing, bioinformatics, flow cytometry and in vivo imaging microscopy.
- Whyte, CE et al., ACKR4 restrains antitumor immunity by regulating CCL21. Journal of Experimental Medicine
Whether you're still at high school or planning to join us mid-year, taking a break from study or rethinking your career path, come chat with us at our STEM Careers Night.
You and your parents are invited to join us on campus on Tuesday 18 May 2021 to see what’s available in the world of STEM.