Understanding T cells in autoimmune disease
Join the Chemokine Biology Lab for a science honours project investigating transcriptional control of inflammatory T helper cells in autoimmunity.
CD4+ T cells play a critical role in autoimmune inflammation such as multiple sclerosis (MS) by producing pro-inflammatory cytokines in response to recognition of tissue antigens.
Recent data indicate that Th cells that co-produce the cytokines GM-CSF and IL-17A are potently inflammatory, but little is known regarding the development of these cells in vivo.
RNA sequencing of these cells has highlighted 2 transcription factors that are associated with this subset of T cells, namely RUNX2 and ID2. In this project we will utilise novel conditional knock-out mice that lack either Runx2 or Id2 in mature T cells to determine the role of these two transcription factors in inflammatory Th cell development and function.
The project will involve mouse models of autoimmunity, in vitro T cell activation cell culture, multi-parameter flow cytometry, RNA sequencing, RT-qPCR and molecular biology.
- Kara, EE et al., CCR2 defines in vivo development and homing of IL-23-driven GM-CSF-producing Th17 cells, Nature Communications
- McKenzie, DR et al., IL-17-producing γδ T cells switch migratory patterns between resting and activated states, Nature Communications
Whether you're still at high school or planning to join us mid-year, taking a break from study or rethinking your career path, come chat with us at our STEM Careers Night.
You and your parents are invited to join us on campus on Tuesday 18 May 2021 to see what’s available in the world of STEM.